Fabrication of micro-structures for optically driven micromachines using two-photon photopolymerization of UV curing resins

Two-photon photopolymerization of UV curing resins is an attractive method for the fabrication of microscopic transparent objects with size in the tens of micrometers range. We have been using this method to produce three-dimensional structures for optical micromanipulation, in an optical system based on a femtosecond laser. By carefully adjusting the laser power and the exposure time we were able to create micro-objects with well-defined 3D features and with resolution below the diffraction limit of light. We discuss the performance and capabilities of a microfabrication system, with some examples of its products.Comment: 12 pages, 10 figure

Measurement of refractive index of single microparticles

The refractive index of single microparticles is derived from precise measurement and rigorous modeling of the stiffness of a laser trap. We demonstrate the method for particles of four different materials with diameters from 1.6 to 5.2 microns and achieve an accuracy of better than 1%. The method greatly contributes as a new characterization technique because it works best under conditions (small particle size, polydispersion) where other methods, such as absorption spectroscopy, start to fail. Particles need not be transferred to a particular fluid, which prevents particle degradation or alteration common in index matching techniques. Our results also show that advanced modeling of laser traps accurately reproduces experimental reality

Orbital Angular Momentum (OAM) of Rotating Modes Driven by Electrons in Electron Cyclotron Masers

The well-defined orbital angular momentum (OAM) of rotating cavity modes operating near the cutoff frequency excited by gyrating electrons in a high-power electron cyclotron maser (ECM)-a gyrotron-has been derived by photonic and electromagnetic wave approaches. A mode generator was built with a high-precision 3D printing technique to mimic the rotating gyrotron modes for precise low-power measurements and shows clear natural production of higher-order OAM modes. Cold-test measurements of higher-order OAM mode generation promise the realization towards wireless long-range communications using high-power ECMs

Spatially-resolved rotational microrheology with an optically-trapped sphere

We have developed a microrheometer, based on optical tweezers, in which hydrodynamic coupling between the probe and fluid boundaries is dramatically reduced relative to existing microrheometers. Rotational Brownian motion of a birefringent microsphere within an angular optical trap is observed by measuring the polarisation shifts of transmitted light. Data gathered in this manner, in the strongly viscoelastic fluid Celluvisc, quantitatively agree with the results of conventional (bulk) rheometry. Our technique will significantly reduce the smallest sample volumes which may be reliably probed, further extending the study of rare, difficult to obtain or highly nonhomogeneous fluids

A photon-driven micromotor can direct nerve fibre growth

Axonal path-finding is important in the development of the nervous system, nerve repair and nerve regeneration. The behaviour of the growth cone at the tip of the growing axon determines the direction of axonal growth and migration. We have developed an optical-based system to control the direction of growth of individual axons (nerve fibres) using laser-driven spinning birefringent spheres. One or two optical traps position birefringent beads adjacent to growth cones of cultured goldfish retinal ganglion cell axons. Circularly polarized light with angular momentum causes the trapped bead to spin. This creates a localized microfluidic flow generating an estimated 0.17 pN shear force against the growth cone that turns in response to the shear. The direction of axonal growth can be precisely manipulated by changing the rotation direction and position of this optically driven micromotor. A physical model estimating the shear force density on the axon is described